VALIDATE RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF LEVOCLOPERASTINE FENDIZOATE PHARMACEUTICAL DOSAGE FORM

Abstract

A simple, accurate, precise method was developed for the simultaneous estimation of the levocloperastine hydrochloride in suspension dosage form. Chromatogram was run through symmetry (250 × 4.6) mm; 5 micron, C18. Mobile phase containing buffer (pH 3.5) and acetonitrile in the ratio of 50:50 was pumped through column at flow rate of 1 ml/min. The mobile phase temperature was maintained at 30 °C, optimized wavelength for levocloperastine is 273 nm. Retention time of Levocloperastine was found to be 5.403 min, tailing factor and number of theoretical plate were 1.146 and 4455 respectively. Regression value of levocloperastine from linearity curve was found to be 0.997109 which suggest very strong linearity , expected in pharmaceutical and QC lab.